Companies Removed since 2003:
| Business | State | Founded | Notes |
| Miller Farm | Delaware | 1684 | Company’s existence could not be confirmed. |
| Cooke Farm | Connecticut | 1720 | Company’s existence could not be confirmed. |
| Sawyer Bentwood Co. | Vermont | 1801 | Sixth-generation owner passed away; company bought by George Campo. |
| Ratcliffe Farms | Mississippi | 1821 | Company’s existence could not be confirmed. |
| Delaware Gazette | Ohio | 1834 | Acquired by Brown Publishing Company of Cincinnati. |
| Fletcher Family Farm | Illinois | 1843 | Company’s existence could not be confirmed. |
| R.C. Perine & Son | Kansas | 1856 | Not continuously owned by one family; closed in 2005. |
Superseded by newly discovered companies:
| BusinessStateFounded |
| Monarch Hydraulics Inc.Michigan1856 |
| Laufersweiler Funeral HomeIowa1856 |
| Iwan Ries & Co.Illinois1857 |
| Klein ToolsIllinois1857 |
| Gundlach-Bundschu WineryCalifornia1858 |
| Baird & WarnerIllinois1860 |
| W.A. Bean & SonMaine1860 |
| Anheuser-Busch Cos.Missouri1860 |
| John Boyle & Co.North Carolina1860 |
| J.H. Home & Sons Inc.Massachusetts1860 |
| Haworth FarmIdaho1861 |
| BacardiPuerto Rico1862 |
| Daynes MusicUtah1862 |
| R.R. Donnelley & SonsIllinois1864 |
| Cargill Inc.Minnesota1865 |
| Milliken & Co.South Carolina1865 |
| WawaPennsylvania1865 |
| Russ Casson & Sons MeatsIowa1865 |
| Destilería SerrallésPuerto Rico1865 |
| Langlois PianosWashington1865 |
| J. Henry Stuhr Inc.South Carolina1865 |
| Newman GalleriesPennsylvania1865 |